Table 1. Tumorigenic potential of shLuc- or shGal-3-infected monolayers or spheres from H1299 cell line.
No. of injected cells | No. of mice with tumor formation/Total No. of mice with cell injection | |
---|---|---|
Monolayers | ||
H1299/shLuc | 1 × 104 | 0/4 |
1 × 105 | 1/4 | |
1 × 106 | 4/4 | |
H1299/shGal-3 | 1 × 104 | 0/4 |
1 × 105 | 0/4 | |
1 × 106 | 2/4 | |
Spheres | ||
H1299/shLuc | 1 × 104 | 3/4 |
1 × 105 | 4/4 | |
1 × 106 | 4/4 | |
H1299/shGal-3 | 1 × 104 | 0/4 |
1 × 105 | 1/4 | |
1 × 106 | 3/4 |
Notes: lentiviral shRNA infected cells were dissociated and subcutaneously injected in NOD/SCID mice (n = 4 per group). The tumor formation was detected 10 weeks after injection.