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. 2014 Dec 31;6(7):5102–5117. doi: 10.18632/oncotarget.3244

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Copyright: © 2015 Matsushita et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A) Flow cytometry of thymic lymphoma cells in FIR^+/+P53^−/− or FIR^+/−P53^−/− mice are indicated. CD4⁺ cells of FIR^+/+P53^−/− (upper column 1) has two populations indicated c-Myc-high (1a) and c-Myc–low intensity (1b). Notably, c-Myc-high (1a) population, presumably lymphoma cells, has c-Myc-low peak, indicating non-tumor cells cluster. CD4⁺CD8⁺ cells (2c) also indicated c-Myc-high intensity population. CD4^low+CD8⁺ (3d) showed c-Myc-high intensity population indicated by FACS (bottom). Red line: Thymus cells of wild type FIR^+/+P53^+/+ mouse. Thin blue line: non-tumor cells of FIR^+/+P53^−/− or FIR^+/−P53^−/− mice. Orange line: Tumor cells of FIR^+/+P53^−/− or FIR^+/−P53^−/− mice. (B) Inverse correlation with significance between c-myc and FIR mRNA expression in CD4^low+CD8⁺ or CD4⁺CD8⁺thymus lymphocytes obtained from FIR^+/+P53^−/− or FIR^+/−P53^−/− mice. Relative c-myc (or FIR)/HPRT mRNA expression of CD4^low+CD8⁺ thymic lymphoma/leukemia cells of FIR^+/+p53^−/− (light green) was 8.6 (0.81), thymic lymphoma cells of FIR^+/−p53^−/− (yellow) was 10.7 (0.58), and thymic lymphoma/leukemia cells of FIR^+/−p53^−/− (blue) was 18.0 (0.37) times as compare to thymic cells of wild mouse (dark orange), respectively (right). The relationship between c-myc/HPRT mRNA (x-axis) and FIR/HPRT mRNA (y-axis) was y=-0.4557x+0.093 (R²=0.9503). Relative c-myc (or FIR)/HPRT mRNA expression of CD4⁺CD8⁺ thymic lymphoma cells of FIR^+/+p53^−/− (light green) was 3.3 (0.56), thymic lymphoma/leukemia cells of FIR^+/+p53^−/− (yellow) was 6.1 (0.48), and thymic lymphoma/leukemia cells of FIR^+/+p53^−/− (blue) was 5.2 (0.56) times as compare to thymic cells of wild mouse (dark orange), respectively. The relationship between c-myc/HPRT mRNA (x-axis) and FIR/HPRT mRNA (y-axis) was y=-1.1161x+0.086 (R²=0.8131). (C) (D) c-myc mRNA and Notch1 mRNA expression was positively correlated each other in sorted thymic lymphoma cells extracted from mice of different genetic backgrounds.