Figure 3. In vitro evaluation of the impact of ETV1 or ETV4 silencing in anchorage-independent growth.

(A–D) Qualitative visualization (left) and quantitative analysis (right) of the several cell line models with manipulated ETV1 or ETV4 expression: MDA-PCa-2b (A), PC3 (B), LNCaP (C), and PNT2 (D) cell lines. Results are shown for each silenced cell population relative to the shNeg cells from three independent experiments. Statistically significant p values are showed by an asterisk (*p < 0.05; **p < 0.01).