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. 2015 Feb 4;6(7):5253–5262. doi: 10.18632/oncotarget.2867

Figure 4. CIP2A siRNA knockdown caused G1 arrest and decreased Cdk1 and Cdk2 protein levels in E7-expressing cells.

Figure 4

(A) Flow cytometry of cells expressing 16E7 transfected with CIP2A siRNA for 48 hr, treated with DMSO control or bleomycin (10 μg/mL) for 24 hr, then stained with PI. G1, S and G2 phases are indicated. (B) Western blot analysis of Cdk1, Cdk2, Cyclin A2, Cdk4, Cdk6, Cyclin D1 protein levels in cells expressing HPV-16E7 transfected with CIP2A siRNA. Babe, vector control.