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. 2015 Jan 20;6(7):5342–5353. doi: 10.18632/oncotarget.3028

Figure 4. Knockdown of PrPc inhibited the HIF-1α-mediated PrPc expression.

Figure 4

(A, B) HCT116 cells were pre-treated 20 nM PrPc RNAi for 24 h and pre-exposed to DEF or hypoxia for 24 h and then detected the protein levels of HIF-1α, PrPc. β-actin was used as a loading control. (C, D) The mRNA levels were determined by quantitative real-time PCR for HIF-1α and PrPc. This treatment is as described in C. These results are representative of three independent experiments. **p < 0.01 compared to basal conditions.