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. 2015 Jan 20;6(7):5342–5353. doi: 10.18632/oncotarget.3028

Figure 5. Knockdown of PrPc blocks protection of hypoxia in TRAIL-treated cells.

Figure 5

HCT116 cells were pre-treated 20 nM PrPc RNAi for 24 h and pre-exposed to DEF or hypoxia for 24 h and further incubated with recombinant TRAIL (200 ng/ml) for an additional 3 h under the same conditions. (A, D) Treated cells were photographed with a light microscope (× 200). (B, C, E, F) Viable cells were stained with crystal violet. Viability of control cells was set at 100%, and viability relative to the control was estimated. These results are representative of two independent experiments. (G) Prnp gene in HCT116 cells was knocked down (PrPc siRNA) using the PrPc siRNA oligomer and then exposed to hypoxic conditions with or without TRAIL 200 ng/ml for 24 h. Cell viability was measured by PI staining assay, (H) Bar graph indicates the number of total cells. HCT116 cells viability was measured by PI staining assay. Percent of numerical value represents the population of apoptotic cells. (E, F) Bar graph indicates the number of total cells and percent of apoptotic cells. *p < 0.05, **p < 0.01: compared to basal conditions.