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. 2015 Jan 20;6(7):5342–5353. doi: 10.18632/oncotarget.3028

Figure 8. PrPc overexpression inhibits TRAIL-induced apoptosis.

Figure 8

Transfection of Ad-Prnp at multiplicity of infection (MOI) 0, 10, 20, 80with Ad-empty at multiplicity of infection 80 for 24 hand incubated with 200 ng/ml TRAIL for 3 h. (A) Treated cells were photographed with a light microscope (× 200). (B, C) Viable cells were stained with crystal violet. Viability of control cells was set at 100%, and viability relative to the control was estimated. These results are representative of two independent experiments. (D) Western blot analysis indicated that overexpression of PrPc was investigated in transfected HCT116 cells after 24 h using the specific anti-PrPc antibody 3F4. *p < 0.05, **p < 0.01 : compared to basal conditions.