Figure 3. JNK1 and SFKs mediate the IL-3-induced mitogenic signaling.

(A–C) BMMCs were pre-treated with SP600125. Cells were probed with [H³]thymidine (A) or PI (B, C) and were analyzed by β-counting (A) or flow cytometry (B, C). (D, E) Wt, Jnk1^−/− (D) or Jnk2^−/− (E) BMMCs were stimulated with IL-3. Cells were probed with [H³]thymidine and analyzed by β-counting. (F, G) BMMCs were pre-treated with SU6656 and were stimulated with IL-3. Cells were probed with [H³]thymidine and analyzed by β-counting (F) (p < 0,001) or lysates were analyzed by westernblotting (G).