Figure 6. Survival of V2D1 cells depends on the IKK-mediated IL-3 production.

(A) V2D1 cells were cultured in IL-3-free medium for different time periods. Lysates were analyzed by westernblotting. (B–D) V2D1 cells were treated with the IKK-inhibitor VII. Lysates were analyzed by westernblotting (B) or cells were probed with [H³]thymidine (C) or PI (D). Cells were analyzed by β-counting (C) or by flow cytometry (D) (C; p < 0,001). (E) V2D1 cells were treated with the IKK-inhibitor VII and collected supernatants were analyzed for IL-3 (p < 0,001). (F) V2D1 cells were treated with the IKK-inhibitor VII and stimulated with IL-3. Cells were probed with [H³]thymidine and were analyzed by β-counting (p < 0,01).