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. 2015 Jan 23;6(7):5412–5425. doi: 10.18632/oncotarget.3027

Figure 2. Inflammation-dependent downregulation of miR-128, miR-134, and miR-330 during murine CAC progression and an inverse correlation with the levels of Mmp3, Mmp10, and Mmp13.

Figure 2

(A) Venn diagram displaying the prediction of murine miRNAs targeting Mmp3, Mmp10, and Mmp13 mRNAs, respectively. (B) qRT-PCR was performed to confirm the expression of the selected miRNAs in mouse colitis tissue, CAC tissue, and normal colon tissue. Several miRNAs including the seven shown here were downregulated in mouse colitis tissue and CAC tissue compared with normal colon tissue. Relative expression of each miRNA was determined with the 2−ΔΔCt method. U6 was used as the internal control, and the expression values for “normal” tissue were set as 1. (C) MiRNA mimics or inhibitors were transfected into CT26.WT cells, and the expression of Mmps was determined by qRT-PCR. Gapdh was used as the internal control, and the expression values of “mimics or inhibitor control” were set as 1. (D) Transfection with miR-128, miR-134, or miR-330 mimic decreased Mmp3, Mmp10, and Mmp13 protein levels, respectively, whereas transfection with the miR-128, miR-134, or miR-330 inhibitor increased Mmp3, Mmp10, and Mmp13 levels, respectively, in CT26.WT cells. (E) Relative expression of Mmp3, Mmp10, and Mmp13 and of miR-128, miR-134, and miR-330 in LPS-stimulated murine macrophages (RAW264.7) revealed a negative correlation based on Spearman analysis (r = –0.58, p < 0.01; r = −0.65, p < 0.001; r = −0.67, p < 0.001). All data are shown as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 compared with control.