(
A) The expression of the
Tg(shh:eGFP) transgene marks the onset of neurogenesis (white arrow) in the developing retina (
Shkumatava et al., 2004) and its expression is not altered in
kif5aa mutant retinae. (
B) RGC differentiation marked by
Tg(pou4f3:mGFP) (
Xiao et al., 2005) expression is not affected. (
C) The glycosylphosphatidyl inositol (GPI)-anchored protein of the immunoglobulin (Ig) superfamily Tag-1 I is expressed in nasal RGCs (
Lang et al., 2001). In situ staining shows that nasal patterning is identical in wild-type and
kif5aa mutant retinae. (
D) Pax2.1, whose expression is restricted to the optic stalk and retinal cells around the choroid fissure and that mediates optic stalk and chiasm formation in fish (
Krauss et al., 1991;
Puschel et al., 1992) shows a normal expression pattern in
kif5aa mutants. (
E) Cryosection of
Tg(Isl2:Gal4, UAS:eGFP) transgenic wild-type and mutant retinae and immunostaining against eGFP (RGCs) (
Ben Fredj et al., 2010), Parvalbumin (marking amacrine cells) (
Godinho et al., 2005) and protein kinase C (marking bipolar cells) (
Godinho et al., 2005) shows that all cell types are present in the right retinal layer. Blue = DAPI. Scale bar = 10 μm. RGCL = RGC layer, AC = amacrine cells, BPC = bipolar cells.