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. Author manuscript; available in PMC: 2016 Feb 18.
Published in final edited form as: Sci Transl Med. 2015 Feb 18;7(275):275ra22. doi: 10.1126/scitranslmed.aaa4963

Fig. 4. In vitro comparison of humanized 2173 EGFRvIII-specific CAR to cetux-CAR.

Fig. 4

(A) Membrane-bound CAR recognition of soluble EGFRvIII or EGFR recombinant proteins. T cells that were either UTD or transduced with the indicated CARs were incubated with soluble biotinylated ECDs of EGFR or EGFRvIII. Histogram plots are shown with gates indicating the percentage of cells that stained with streptavidin-PE. (B) Proliferation of UTD or T cells transduced with the indicated CAR T cells after 4 days of coculture with BHK cells expressing EGFRwt or EGFRvIII. Y axis indicates fold proliferation over CAR T cells stimulated with parental BHK cells. (C) ELISA-based cytokine analysis on supernatants collected 24 hours after stimulation of T cells with BHK, BHK-EGFRwt, or BHK-EGFRvIII cells.