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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Mol Microbiol. 2015 Feb 26;96(2):388–404. doi: 10.1111/mmi.12943

Figure 7. Staphopain activity is upregulated in rot mutant.

Figure 7

Strain LAC (WT), Δrot mutant, and Δrot complemented strains were grown in TSB and samples were collected during late log phase (4 hr) and stationary growth phase (24 hr). Cells were removed by filtration and spent media was saved for enzyme assays and immunoblots. A. Protease activity using a general protease FRET substrate at 24 hr. B. Immunoblot for SspA at 24 hr. C. Measure of ScpA activity using the ScpA-specific FRET substrate at 4 hr. D. Immunoblot for ScpA at 4 hr. E Measure of SspB activity using a pNA substrate at 24 hr. F. Immunoblot for SspB at 24 hr.