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. 2015 Apr 24;4(6):731–742. doi: 10.1242/bio.201410884

Fig. 1. VEGF-A isoform-specific stimulated endothelial cell signalling.

Fig. 1.

(A) Schematic depicting VEGF-A stimulated VEGFR2 phosphorylation, activation of downstream signalling pathways linking to cellular responses. Abbreviations: p38, p38 mitogen-activated protein kinase; HSP27, heat-shock protein of 27 kDa; Akt, Protein kinase B; eNOS, endothelial nitric oxide synthase; NO, nitric oxide; MEK1, mitogen-activated protein kinase 1; ERK1/2, p42/44 mitogen-activated protein kinase. (B) Endothelial cells subjected to different VEGF-A165 or VEGF-A121 concentrations (0, 0.025, 0.25 or 1.25 nM) for 5 or 15 min were lysed and processed for immunoblot analysis using phospho-specific antibodies against p-VEGFR2, p-eNOS, p-p38 and p-ERK1/2. (C–J) Quantification of VEGF-A isoform-specific signal transduction events. Quantification of (C,D) VEGFR2-pY1175, (E,F) eNOS-pS1177, (G,H) p38 MAPK-pT180/pY182 or (I,J) ERK1/2-pT202/Y204 upon (C,E,G,I) VEGF-A165 or (D,F,H,J) VEGF-A121 stimulation. Error bars indicate ±SEM (n≥3). p<0.05 (*), p<0.01 (**), p<0.001 (***), p<0.0001 (****).