Fig. 4. VEGF-A isoform-specific Ca²⁺ flux promotes differential NFATc2 activation.

(A) Schematic depicting VEGF-A-stimulated cytosolic calcium ion rise and effects on NFATc2 dephosphorylation and nuclear translocation. (B) Endothelial cells subjected to stimulation with 0.25 nM VEGF-A₁₆₅ or VEGF-A₁₂₁ for 5, 15, 30 and 60 min were lysed and process for immunoblot analysis to detect phospho- (inactive) and dephospho- (active) NFATc2 species. (C) Quantification of relative active versus inactive NFATc2 levels upon stimulation with VEGF-A₁₆₅ and VEGF-A₁₂₁. Error bars indicate ±SEM (n = 3). p<0.05 (*), p<0.001 (***).