Fig. 5. VEGF-A isoforms promote differential NFATc2 nuclear translocation.

(A) Endothelial cells stimulated with 0.25 nM VEGF-A₁₆₅ or VEGF-A₁₂₁ for 5, 15 and 30 min were fixed and processed for immunofluorescence microscopy using rabbit anti-NFATc2 (green); nuclei stained using DAPI (blue). Scale bar, 1000 µm. (B) Quantification of NFATc2 nuclear co-distribution at 0, 5, 15 and 30 min after stimulation with VEGF-A₁₆₅ or VEGF-A₁₂₁. Error bars indicate ±SEM (n = 3). p<0.0001 (****).