Fig. 7. Mechanism for VEGF-A isoform-specific regulation of endothelial cell migration.

Schematic depicting VEGF-A isoform-specific regulation of NFATc2 activation and regulation of endothelial cell migration. Numbered steps denote: (1) VEGF-A isoforms promote differential recruitment and activation of PLCγ1 through interaction with VEGFR2; (2) due to increased PLCγ1 activation, VEGF-A₁₆₅ promotes an increased rise in intracellular Ca²⁺, versus VEGF-A₁₂₁; (3) Increased VEGF-A₁₆₅ stimulated intracellular Ca²⁺ flux results in increased NFATc2 dephosphorylation, versus VEGF-A₁₂₁; (4) dephosphorylated NFATc2 translocates into the nucleus where it regulates endothelial gene transcription; (5) VEGF-A-stimulated NFATc2 regulated gene expression promotes endothelial cell migration.