Fig. (1).

The translated amino acid sequences (10,465) of FANTOM clones were divided into two equal parts; onehalf from the 5’ end of the mouse genome (black) and the other half from the 3’ end of the genome (gray). The amino acid compositions encoded by the first 5, 10, 50, 100, 500, and 1,000 genes in each half were analyzed. There were 5,232 and 5, 233 genes in the first and second halves, respectively. The value of each amino acid was expressed as the percentage of the total number of amino acids and all values were visualized on a radar graph. In our previous studies [11, 26], cell homogenates were hydrolyzed in 6N HCl at 105°C for 24 h, and then the cellular amino acids were analyzed using a Hitachi L8500A amino acid analyzer (Hitachi, Tokyo, Japan). In the previous analysis, the glutamine and asparagine residues were converted to glutamic acid and aspartic acid, respectively; thus, the values for the acidic forms represent the sums of both forms. In the present study, the values that were used represent the sums of both values. Tryptophan was omitted in our previous [11, 26] and present studybecause this amino acid decomposed during hydrolysis and its value was 1% of the total codon frequency, based on 35 human genes [42]. This figure first appeared in Natural Science, 2010, 2 (10), 1104-1112, and is reproduced with permission.