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Effects of demethylation agents on cell proliferation and invasion. Cell proliferation was inhibited by demethylation agents in (A) Saos-2 and (B) MG63 cells. Cell invasive ability was reduced by demethylation agents in (C) Saos-2 and (E) MG63 cells. Quantification of the Transwell™ assay in (D) Saos-2 and (F) MG63 cells. Data are expressed as mean ± standard deviation. *P<0.05 and **P<0.01 vs. control (Con). Aza, 5-Aza-2-deoxycytidine; PBA, 4-phenylbutyric acid.
