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. 2014 Apr 16;6(8):5567–5581. doi: 10.18632/oncotarget.1901

Figure 2. RCC cancer spheres are enriched in the CD24+ cell population.

Figure 2

(A) Parental CAKI-1 and ACHN cells and their sphere derivatives established in SFDM and or by anti-miR-17 transfection were subjected for flow cytometric analysis to detect CD24 and CD44 cell surface proteins. CD24+/CD44+ ratio was significantly higher in SFDM spheres. Spheres derived from anti-miR-17 transfection showed a further increase in the double positive cell population. Cd44 and Cd24 expression was also assessed by immunocytochemistry. ACHN spheres stained positive for CD44 and CD24 (B-D) but adherent ACHN cells appears to be CD24 negative (E-F).