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. 2014 Apr 16;6(8):5567–5581. doi: 10.18632/oncotarget.1901

Figure 6. miR-17 inhibition results in formation of RCC spheres.

Figure 6

(A) CAKI-1 and ACHN cells were kept untreated in SFDM medium, were treated with the transfection agent siPORT or were transiently transfected with anti-miR-17. Sphere formation was followed for 6 days. (B) Spheres were dissociated with trypsin and secondary sphere formation capacity was assessed. RPP: random pool of miRNA precursors.