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. 2015 Feb 28;6(8):5818–5831. doi: 10.18632/oncotarget.3461

Figure 4. GZMM promotes cloning forming capacity, invasiveness and cytokine secretion in vitro.

Figure 4

(A-B) 300 or 500 cells were seeded in 60mm-plates in quadruplicate with complete medium and cultured for one week at 37°C. After cultured, cells were fixed, stained with Giemsa and counted as shown. (C-D) The secreted murine VEGF and IL-6 protein levels in the supernatants of CT26-NC, CT26-sh GZMM, 4T1-NC, and 4T1-GZMM cells were measured by an ELISA kit. (E-F) The cells were seeded in the upper chamber with matrigel. After incubation for 20 h, the cells that migrated to the lower surface of the membrane were counted, as shown in the figure. The data are expressed as the mean values ± SEM from one experiment performed in triplicate from three independent experiments. **p<0.01, ***p<0.001.