Figure 1. High-throughput colony formation screen identifies TPK1 as novel radiosensitizer.

A) Crystal violet stained 96-well plates following 0 Gy and 7 Gy irradiation. The TPK1 siRNA containing well is indicated. Representative plates of quadruplicate repeats are shown. Plate layout can be found in Supplementary Figure S1. B) Heatmap of the R-scores of the primary screen of 709 Dharmacon Kinome ON-TARGETplus siRNA library. Screen was carried out twice and rank product scores were calculated based on R-score. Radiosensitizing siRNAs shown by R-scores <0. Top 25 lowest and highest ranked siRNAs are shown. siRNAs that reduced plating efficiency below 5% were excluded from analysis. C) Heatmap of secondary screen of the top 76 targets from primary screen using Dharmacon siGENOME siRNA. Screen was performed twice and ordered based on rank product.