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. 2015 Feb 28;6(8):5978–5989. doi: 10.18632/oncotarget.3468

Figure 2. Validation of TPK1 as a target for tumor specific radiosensitization.

Figure 2

A) CFA of tumor lines HeLa, SQ20B and BT-549 cells irradiated following Ambion siRNA transfection with non-targeting (siNT) and siTPK1. HeLa and BT-549 cells were transfected with 40 nM, SQ20B with 20 nM siRNA. HeLa cells were transfected with two different TPK1 siRNAs: TPK1-1 and siTPK1-2. Knockdown was confirmed by immunoblotting (insets). B) CFA of normal fibroblast HFL-1 and MRC5 cells irradiated following 20 nM (HFL-1) or 40 nM (MRC5) Ambion siRNA transfection with siNT and siTPK1. Knockdown was confirmed by immunoblotting (insets). CFA: representative of three independent experiments, data points presented as mean +/− SD from triplicate wells, p-values generated by factorial 2-way ANOVA. Sensitization enhancement ratio at 0.10 surviving fraction (SER10) calculated from non-linear regression of linear quadratic model.