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. 2015 Feb 28;6(8):6001–6013. doi: 10.18632/oncotarget.3469

Figure 4. The effects of ectopicCir-ITCH expression on ESCC cell cycle and proliferation.

Figure 4

(A) Cell cycle analysis of Eca-109 and TE-1 cells after transfected with Cir-ITCH, or Control lentiviruses. Flow cytometry data are represented as mean±SEM and are based on three independent experiments. (B) Eca-109 and TE-1 cells were seeded in 96-well plates after transfected with Cir-ITCH and Control lentiviruses, and cell proliferation was performed daily for 3 days using the CCK-8 assay. Six replicates for each group and the experiment repeated three times. Data are mean±SEM. *P<0.05 compared with controls. (C) Representative colony formation assay in Eca-109 and TE-1 cells after transfected with Cir-ITCH and Control lentiviruses, the numbers of colonies in vector-transfected controls were set to 100%. Data are present as mean±SEM from three experiments. (D) Representative images of xenografts in each group 2 weeks after subcutaneously implanted ESCC cells stably expressing Cir-ITCH and respective empty vector (left). Mean tumor volumes from eight nude mice of each group are shown at different time points (right). *P<0.05 compared with controls.