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. 2015 Jan 21;6(8):6014–6028. doi: 10.18632/oncotarget.3338

Figure 3. miR-1236 inhibits the migration, invasion and VM of HCC cells and regulates the EMT- and VM-associated molecules.

Figure 3

(A) Transwell migration assays were performed to detect the migration ability of HepG2 and QGY-7703 cells transfected with pcDNA3/pri-miR-1236, ASO-miR-1236 or the corresponding controls. (B) Transwell (coated with Matrigel) invasion assays were performed to determine the invasion ability of HepG2 and QGY-7703 cells transfected with pcDNA3/pri-miR-1236, ASO-miR-1236 or the corresponding controls. (C) Three-dimensional Matrigel culture assays were performed to detect VM in HepG2 and QGY-7703 cells transfected with pcDNA3/pri-miR-1236, ASO-miR-1236 or the corresponding controls. (D) The influence of miR-1236 on the protein levels of EMT-associated molecules (E-cadherin and vimentin) and of a key VM molecule (VE-cadherin) was determined by western blotting. *p<0.05, **p<0.01, ***p<0.001. All error bars indicate the means±SDs. All experiments were repeated at least three times.