Skip to main content
. 2015 Jan 21;6(8):6014–6028. doi: 10.18632/oncotarget.3338

Figure 6. miR-1236 regulates the PTEN/PI3K/Akt pathway mediated by AFP.

Figure 6

(A) Western blot assays were performed to determine the effect of AFP on the expression of PTEN, p-AKT and AKT in QGY-7703 cells. (B) 293T cells were cotransfected with pA3M1/PTEN (Myc tag) and pcDNA3-flag or pcDNA3-flag/AFP (Flag tag) and then being treated as described in the materials and methods, and the influence of ectopic AFP expression on the ubiquitination levels of PTEN was determined by western blotting. (C) Western blotting was performed to determine the effect of miR-1236 on the expression of AFP, PTEN, p-AKT and AKT in QGY-7703 cells (Left). Western blot assays were performed to detect the restoration of AFP upon presentation of miR-1236 as well as to determine the influence on the expression of PTEN, p-AKT and AKT in QGY-7703 cells (Right). (D) The expression level of miR-1236 in tumor tissues isolated from the nude mice described in Figure 2C (Left). Representative IHC images showing the magnitude of AFP and PTEN expression in tissues isolated from nude mice (Right). **p<0.01, n=3. All error bars indicate the means ± SDs.