Table 1. The primers used in vector consructions.
Name | Primer Sequence |
---|---|
pri-miR-1236 sense | 5′-CGCGGATCCCTGGCCCTCACTTACCTC-3′ |
pri-miR-1236 anti-sense | 5′-CCGAATTCCCATCTACATTCCAACTTGGAG-3′ |
ASO-miR-1236 | 5′-CUGGAGAGACAAGGGGAAGAGG-3′ |
ASO-NC | 5′-CAGUACUUUUGUGUAGUACAA-3′ |
AFP sense | 5′-ACTAGTAGCGGCCGCCAGTGTGCTG-3′ |
AFP anti-sense | 5′-CTCCGGATCCTAGGTGACACTATAGAATAGG-3′ |
AFP-shR-Top | 5′-GTACAAGGAAGTAAGCAAAATGTTCAAGAGACATTTTGCTTACTTCCTTGTA-3′ |
AFP-shR-Bottom | 5′-AAAATACAAGGAAGTAAGCAAAATGTCTCTTGAACATTTTGCTTACTTCCTTGTAC-3′ |
AFP-3′UTR sense | 5′-CACGGATCCAACTTGAGGCTGTCATTGC-3′ |
AFP-3′UTR anti-sense | 5′-CGGAATTCGATAAGGAAATCTCACATAAAAGTC-3′ |
AFP-3′UTRmut sense | 5′-AAATTACTTCAGGCCATCACAAGACAAAACG-3′ |
AFP-3′UTRmut anti-sense | 5′-CGTTTTGTCTTGTGATGGCCTGAAGTAATTT-3′ |