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. 2015 Jan 31;6(8):6179–6190. doi: 10.18632/oncotarget.3350

Figure 2. miR-148a regulates viral release from cells infected with Ad-L5-8miR148aT.

Figure 2

A, B. Quantification of viral production from supernatants obtained from cells infected with 50 vp/cell of Ad-wt and Ad-L5-8miR148aT at 72h post-infection by (A) hexon immunostaining, (B) qPCR. Data is shown as the mean ± SEM of five (A) and four (B) independent experiments. ** p<0.01 and *** p<0.001. (C). Quantification of viral production in supernatant (extracellular) and pellets (intracellular) of cells infected with 50 vp/cell of Ad-wt and Ad-L5-8miR148aT at 48h post-infection by qPCR. Data is shown as the mean ± SEM of three independent experiments. * p<0.05. (D). Quantification of viral production in supernatant of cells infected with Ad-wt, Ad-E1A-8miR148aT and Ad-L5-8miR148aT upon several passages by qPCR. Data is shown in arbitrary units (AU) as the mean ± SEM of four independent experiments, calculated as the 2ΔCt between the miRNA-targeted viruses and Ad-wt. Differences between slopes were analyzed using F-test for nonlinear models *** p<0.001.