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. 2015 Jan 31;6(8):6179–6190. doi: 10.18632/oncotarget.3350

Figure 5. Ad-L5-8miR148aT shows oncolytic potency in vitro and strong antitumoral activity in RWP-1 xenografts and PDX models.

Figure 5

(A). Cytotoxicity assays in the indicated cell lines. Half growth inhibitory concentration (IC50) was calculated for each cell line from dose-response curves. Data is shown as the mean ± SEM of four independent experiments. (B). Cytotoxic effects of Ad-wt, Ad-E1A-8miR148aT and Ad-L5-8miR148aT supernatants obtained after 7 consecutives passages of amplification in MIA PaCa-2 miR-148a and MIA PaCa-2 miR-SC cells. Representative image obtained by methylene blue staining of the culture. (C). RT-qPCR expression of miR-148a from non-tumor human pancreas (n=11) and from RWP-1 xenograft (n=4) and CP13 (n=4), CP15 (n=4) PDX. (D). Follow-up of tumor volumes. RWP-1 xenografts were treated intratumorally with a single injection of 5×1010vp/tumor Ad-L5-8miR148aT (n=8), Ad-E1A-8miR148aT (n=8) and Ad-wt (n=8) or with saline (n=8). ** p<0.01. E. Follow-up of tumor volumes. CP13 PDX were treated intratumorally with a single injection of 5×1010vp/tumor Ad-L5-8miR148aT (n=10) or with saline (n=10). *** p<0.001. F. Follow-up of tumor volumes. CP15 PDX were treated intratumorally (n=12) or intravenously (n=10) with a single injection of 5×1010vp/tumor Ad-L5-8miR148aT or with saline (n=10). *** p<0.001.