Figure 4. EGFR^L858R-activated signaling molecules in transgenic mice.

Transgenic mice were fed with Dox diet for 2-8 weeks. Lung tissues were flushed twice with 10 ml PBS and snap-frozen in liquid nitrogen. Frozen tissues were thawed and lysed with the lysis buffer. Equal amount of proteins in Triton X-100 soluble supernatants were analyzed by immunoblotting with indicated antibodies. W, wildtype, B, C/EL858R bitransgenic, T, C/S65/EL858R or C/S669/EL858R tritransgenic.