Figure 5. Lung hyperproliferative lesions in transgenic mice.

A, H&E sections of lungs from transgenic mice. Transgenic mice were fed with Dox diet for 2-8 weeks. Lungs were collected and processed as described in Materials and Methods. H&E stained slides were scanned with a ScanScope XT slide scanner (Aperio). Examples of H&E stained sections at each time point after Dox induction from bitransgenic and two different tritransgenic lines are presented. Top panels from each transgenic line show images of whole lung sections. The bottom panels from each transgenic line show a higher magnification (200x) of the boxed areas. B, Box-and-Whiskers plot of the extent of hyperproliferative lesions in the lungs of Dox-induced transgenic mice. Three 4 μm sections that were 25 μm apart from each lung paraffin block were stained with H&E. Images were acquired and analyzed using a histology pattern recognition algorithm (Aperio). Areas of hyperproliferative lesions were calculated. Numbers on each column indicate the number of mice analyzed in that set of samples. *, p <0.05. Hyperproliferative lesions include atypical adenomatous hyperplasia, non-mucinous adenocarcinoma with lepidic pattern, bronchioloalveolar adenoma, and solid adenoma (<0.5 mm in diameter) and adenocarcinoma (>0.5 mm in diameter).