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. 2015 Jan 31;6(8):6251–6266. doi: 10.18632/oncotarget.3360

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Copyright: © 2015 Bhandary et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A) Suspended BT549 and Hs578T cells stained with CellMask orange and scored blindly for the presence of McTNs show that 10μM Y-27632 treatment for 1 hour significantly increases the frequency of McTNs compared to vehicle-treated cells in both the cell lines tested. Treatment with 50μM colchicine, a microtubule-depolymerizing drug, significantly decreases McTN formation when used singly or in combination with Y-27632. Columns represent the mean +/− SD of three independent experiments in each of which 100 cells were scored in triplicate (900 cells total for every treatment group) (p<0.05, t-test, black asterisks). B) Representative live-cell confocal microscope maximum intensity projection images of CellMask orange stained suspended BT549 cells show more cells with McTNs in Y-27632-treated group compared to the vehicle control group (arrows) (60x magnification). C) 4μg/ml Rho Activator II treatment for 3 hours decreases McTN formation in BT549 and Hs578T cells. Columns represent the mean +/− SD of three independent experiments in each of which 100 cells were scored in triplicate (900 cells total for every treatment group) (p<0.05, t-test, black asterisks) D) Representative live-cell confocal microscope maximum intensity projection images of CellMask orange stained suspended BT549 cells show loss of McTN formation in Rho Activator II-treated cells compared to vehicle-treated cells (60x magnification).

A) Suspended BT549 and Hs578T cells stained with CellMask orange and scored blindly for the presence of McTNs show that 10μM Y-27632 treatment for 1 hour significantly increases the frequency of McTNs compared to vehicle-treated cells in both the cell lines tested. Treatment with 50μM colchicine, a microtubule-depolymerizing drug, significantly decreases McTN formation when used singly or in combination with Y-27632. Columns represent the mean +/− SD of three independent experiments in each of which 100 cells were scored in triplicate (900 cells total for every treatment group) (p<0.05, t-test, black asterisks). B) Representative live-cell confocal microscope maximum intensity projection images of CellMask orange stained suspended BT549 cells show more cells with McTNs in Y-27632-treated group compared to the vehicle control group (arrows) (60x magnification). C) 4μg/ml Rho Activator II treatment for 3 hours decreases McTN formation in BT549 and Hs578T cells. Columns represent the mean +/− SD of three independent experiments in each of which 100 cells were scored in triplicate (900 cells total for every treatment group) (p<0.05, t-test, black asterisks) D) Representative live-cell confocal microscope maximum intensity projection images of CellMask orange stained suspended BT549 cells show loss of McTN formation in Rho Activator II-treated cells compared to vehicle-treated cells (60x magnification).