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. 2015 Jan 31;6(8):6251–6266. doi: 10.18632/oncotarget.3360

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Copyright: © 2015 Bhandary et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Real-time changes in electrical impedance caused by reattaching A) BT549 and B) Hs578T cells indicate an increase in reattachment efficiency of cells treated with 10μM Y-27632 for an hour compared to vehicle-treated cells. 50μM colchicine treatment for an hour decreases reattachment frequency following detachment when used singly or in combination with Y-27632 in both the BT549 and Hs578T cells. Graph represents mean +/− SD of triplicate values for each treatment group. Three independent experiments were performed. Representative phase contrast images of C) BT549 and D) Hs578T cells show cells reattaching to the plate more efficiently in the Y-27632-treated group compared to the vehicle-treated group (10x magnification) [VC= Vehicle, Col=Colchicine].

Real-time changes in electrical impedance caused by reattaching A) BT549 and B) Hs578T cells indicate an increase in reattachment efficiency of cells treated with 10μM Y-27632 for an hour compared to vehicle-treated cells. 50μM colchicine treatment for an hour decreases reattachment frequency following detachment when used singly or in combination with Y-27632 in both the BT549 and Hs578T cells. Graph represents mean +/− SD of triplicate values for each treatment group. Three independent experiments were performed. Representative phase contrast images of C) BT549 and D) Hs578T cells show cells reattaching to the plate more efficiently in the Y-27632-treated group compared to the vehicle-treated group (10x magnification) [VC= Vehicle, Col=Colchicine].