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Confirmation of target candidates using in vitro bead-based pull-down assay. (A) Flow scheme of the high throughput pulldown assay. (B) Immunoblot analysis of the pulldown. Top panel (pulldown, PD) shows that the query proteins (HaloTag-LidA or -SidM), but not the Tag, were precipitated by GST-bait-coated beads. Bottom panel (western blot, WB) shows detection of the GST-bait protein by immunoblot using anti-GST antibody.
