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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: J Proteome Res. 2015 Mar 18;14(4):1920–1936. doi: 10.1021/pr5013015

Figure 5.

Figure 5

Detection of AMPylated host targets by combining NAPPA and click chemistry. (A) Representative images of AMPylated targets (yellow boxes) on NAPPA slides after incubation with either wild-type SidM or its AMPylation-defective mutant SidMD110/112A, annotated as in Figure 2D. (B) Confirmation of target candidates using bead-based AMPylation assay with wild-type SidM (WT) or SidMD110/112A (D/A). Top panel (Az-rho) shows the detection of AMPylated targets using in-gel fluorescence with az-rho based on click reaction. Bottom panel (WB) is the detection of the host target protein (substrates) by immunoblot.