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. Author manuscript; available in PMC: 2015 Dec 12.
Published in final edited form as: Nat Commun. 2015 Jun 12;6:7389. doi: 10.1038/ncomms8389

Figure 1. Nucleotide metabolism genes are targets of mtp53.

Figure 1

(a) HCC38, BT549 and MDAMB231 cells were transfected with either a control (Ct) or p53 siRNA (p53si) and cell counts and doubling times were determined after 72 hours. Error bars indicate mean ±SD of three independent replicates. Inset is western blot showing p53 knockdown. (b) Chromatin immunoprecipitation was performed on MDAMB231 cells with either a control (IgG) or p53 antibody and real-time PCR was used to detect the presence of the indicated promoter regions. The data was normalized to input DNA. Error bars indicate mean ±SD of two independent replicates. (c) BT549, HCC38 and MDAMB231 cells were infected with an empty vector (EV) or p53 shRNA (p53sh), selected for 3 days and then processed for western blot analysis of the indicated proteins. (d) Rescue experiments were performed by infecting p53 shRNA cells with R280K and R249S expression vectors, selected for 7 days and then processed for western blot analysis of the indicated proteins.