Table 1.

Properties of intact luciferases.

Luciferase	Amino acids	Cofactors	λmax (nm) (in solution)	λ range (nm)	Relative intensity (intracellular)⁽¹⁾	Relative intensity (mouse tissue)⁽¹⁾
Firefly	550	ATP, Mg⁺, O₂	578 at 37° C⁽²⁾	520–740 at 37° C⁽²⁾	1	1
Renilla	311	O₂	475⁽¹⁾	420–580⁽¹⁾	1	ND^*
Gaussia	185 (secreted form)	O₂	480⁽¹⁾	440–590⁽¹⁾	Approx 100	2

Sources

⁽¹⁾

(15)

⁽²⁾

(23)

Reference 1 includes data for secreted Gaussia luciferase in mice compared with Renilla luciferase, but this data is not applicable to intracellular Gaussia complementation assays.