Figure 6. Release of mitochondrial CytC in SH-SY5Y and ECs after 3 days of peptide challenge.
Immunocytochemical evaluation of CytC localization in SH-SY5Y and ECs treated for 3 days with the less aggressive Aβ40 and Aβ(isoD) peptides (50 μM). Green fluorescence depicts CytC immunostaining, as in Figures 4 and 5; punctate staining represents mitochondrial localization, and diffuse green staining indicates cytoplasmic release. Blue fluorescence signal depicts nuclear DNA counterstained with DAPI. Magnification 40×. Cnt, control.