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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: Biochem J. 2013 Dec 15;456(3):347–360. doi: 10.1042/BJ20130652

Figure 9. Confocal microscopy assessment of the effect of methazolamide in preventing Aβ-mediated CytC release and restoring mitochondrial membrane potential in ECs.

Figure 9

ECs, challenged with Aβ-Iowa (50 μM, 16 h) in the presence and absence of methazolamide, were incubated with MitoTracker® Red and further immunostained for CytC, as above. Top panel, green fluorescence depicts CytC; middle panel, red fluorescence illustrates the signal of oxidized MitoTracker®, the localization of which to the mitochondria is dependent on the organelles’ membrane potential; bottom panel, merged images. Cnt, control; M, methazolamide. Magnification 40×.