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. 2015 Jun 6;4:245. doi: 10.1186/s40064-015-1022-7

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© Herrmann and Aronica 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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FXYD3 levels in MCF-7 cells. MCF-7 cells were treated for 48 h with PBS/0.1% ethanol control vehicle, 1 nM estrogen, 10 nM tamoxifen or with estrogen in combination with tamoxifen, and expression of FXYD3 was determined by flow cytometry using anti-FXYD3 antibodies and a Guava PC flow cytometer, as described in the “Methods”. Each bar represents the mean ± SD of FXYD3 fluorescence per cell for six samples obtained in separate experiments. Asterisk significant difference (p < 0.05) from PBS/ethanol-treated control cells.