Table 2.

Serology and real-time PCR results of serum samples collected from animals, which had aborted recently and positive in at least one test

Case no.	Host	History of samples collection after abortion (weeks)	Serological assay			PCRd
			RBTa	CFTb	ELISAc	Bcsp 31	IS711 B. abortus	IS711 B. melitensis
1	Cow	4	+	+	+	+	+	−
2	Cow	3	+	+	+	+	+	−
3	Cow	6	+	+	+	+	+	−
4	Cow	4	+	+	+	+	+	−
5	Cow	3	+	+	+	+	+	−
6	Cow	3	+	+	+	+	+	−
7	Cow	6	+	+	+	+	+	−
8	Cow	4	+	+	+	+	+	−
9	Cow	4	+	+	+	+	+	−
10	Cow	3	+	+	+	+	+	−
11	Buffalo	4	+	+	+	+	+	−
12	Buffalo	1	−	−	−	+	+	−
13	Buffalo	1	−	−	−	+	+	−
14	Buffalo	1	−	−	−	+	+	−
15	Buffalo	1	−	−	−	+	+	−
16	Goat	4	+	+	+	+	+	−
17	Goat	3	+	+	+	+	+	−
18	Goat	4	+	+	+	+	+	−
19	Goat	1	−	−	−	+	+	−
20	Goat	1	−	−	−	+	+	−
21	Goat	1	−	−	−	+	+	−
22	Goat	1	−	−	−	+	+	−
23	Goat	1	−	−	−	+	+	−
24	Goat	1	−	−	−	+	+	−
25	Sheep	4	+	+	+	+	+	+
Total positive			15	15	15	25	25	1

^aConsidered positive when showing any degree of agglutination.

^bPositive samples (≥20 IU/ml).

^cpositive samples showing cut off values (≥2).

^dPositive samples showing ct value (ct ≤ 40).