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. 2015 Jun 4;13:178. doi: 10.1186/s12967-015-0536-7

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© Zhang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Anti-dsDNA antibodies induced NF-κB activation via PERK-eIF2α-ATF4 ER stress pathway. a Depletion of ATF4 by ATF4 siRNA was confirmed by western blot. b HMCs were stimulated with anti-dsDNA antibodies. NF-κB p65 nuclear translocation was measured by western blot analysis of nuclear protein extract. c ATF4 siRNA significantly inhibited NF-κB p65 nuclear translocation induced by anti-dsDNA antibodies. d–f ATF4 siRNA significantly inhibited the expression of IL-1β, MCP-1 and TNF-α mRNA as measured by qPCR. g–i ATF4 siRNA also significantly inhibited the secretion of IL-1β, MCP-1 and TNF-α in supernatants as measured by ELISA. All experiments were repeated for three times. Data were expressed as mean ± SD, *P < 0.01, versus control group, #P < 0.01, versus scramble siRNA group. CTL Control and anti-dsDNA Abs anti-dsDNA antibodies.