Table 1.

Summary of high throughput screening results

Total # compounds screened	20,000
Hits from primary screen	180 (0.9%)
Dose response	36 (0.18%)
Available for re-supply	32 (0.16%)
Confirmed inhibitors	27 (0.14%)
IC50 <30 μM	9 (0.05%)
Enhanced neurite outgrowth	1 (0.005%)

A 20,000 compound library was screened using the ELISA platform as described in Materials and Methods. 180 compounds were subject to dose response analysis. Of these 36 inhibited the Shroom3–ROCK interaction with pIC50 values greater than 4.0, had >60% efficacy at maximum dose tested, and had recovery rates in unrelated screens at <22%. 32 of the 36 chemicals were available for repurchase and of these 27 reconfirmed as inhibitors of the Shroom3–ROCK interaction. Nine compounds of the 27 confirmed hits have IC50 values less than 30 μM. One compound enhances neurite outgrowth.