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Long-term HF–HC–HSD induces hepatic fibrosis. Hepatic mRNA expression of αSMA (a), pro-collagen-1 (b), TGFβ (c), TIMP-1 (d), TIMP-3 (e), MMP2 (f), and MMP-9 (g) were measured using qPCR in male mice at each time point. Representative images of Sirius red staining to evaluate fibrosis at 27 and 49 weeks are shown (h).
