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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Biomaterials. 2015 May 15;59:30–38. doi: 10.1016/j.biomaterials.2015.04.040

Fig. 3.

Fig. 3

Quantification of circulating FITC by anti-FITC-MNs in laser-treated skin. (A) C-MNs (unfilled circle) and anti-FITC-MNs (filled square) were applied into laser-treated skin for indicated times in mice receiving FITC intravenously. Each symbol represents one array consisting of 9 MNs. The cutoff value is indicated by a dashed line and defined as Figure 1D. Inset shows fluorescence images of one anti-FITC-MN array. Note, all MNs in the array show uniform and strong FITC binding. Scale=100 μm. (B). The amounts of circulating FITC measured by anti-FITC-MNs are similar to those obtained with fluorescence spectrophotometer. Anti-FITC-MNs or C-MNs were inserted into laser-treated skin for 30 min in mice receiving varying amounts of FITC intravenously, and the FITC captured on MNs was calculated by FITC intensity in comparison with a standard curve run in parallel.