Fig. 2.

Principle of slits generation and controlling waveform of dual-slit confocal LSM system. (a) Principle of the slit generation by the sCMOS sensor. The bars mean the pixel rows on the sensor. The activate rows move from middle to the both side of the sensor. Arrows in readout sequence plot mean the time sequence of activated rows’ readout on the sensor. Neighboring rows start exposing with a delay. (b)Controlling waveforms of the AOTF, galvanometer mirror and sCMOS. When the AOTF switches the laser on, the galvanometer scans the beam from middle to both sides of the field of view. The sCMOS works at level trigger mode and starts exposing when the galvanometer mirror starts scanning.