Fig. 5.

The effect of various slits’ width on SNR and frame rate of dual-slit confocal LSM. (a) to (e) Images of 200 nm fluorescent beads with the slit width of 6.45 μm, 32.25 μm, 64.5 μm, 290.25 μm and 580.5 μm, respectively. (f) indicates the SNR changes with the different slit’s width. The dots are experimental SNR. The black line is the theoretical value calculated by Eq. (2). The frame rates of single and dual-slit confocal LSM with exposure time of 1 ms at 2048 by 2048 pixels are shown in blue and red line, respectively. Scale bar is 20 μm.