Fig. 8.

Single-neuron resolved activity of zebrafish’s midbrain and hindbrain recorded by dual-slit confocal LSM ( Media 2 ^{(1.5MB, MOV)} ). (a) Brain image of zebrafish larvae (HuC-GCaMP5G, albino, 5 days old). Neurons were segmented manually and colored by different area of brain. Scale bar is 100 μm. (b) Activity traces of all neurons marked in (a). The label denotes the corresponding brain regions in: Left Midbrain (LMB, red), Right Midbrain (RMB, green), Left Hindbrain (LHB, blue) and Right Hindbrain (RHB, yellow). (c) Fluorescence (ΔF/F) traces of different neurons indicated in (b). (d) Close-up views of the calcium transients indicated by the box in (c).