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. Author manuscript; available in PMC: 2016 May 7.
Published in final edited form as: Cell. 2015 May 7;161(4):858–867. doi: 10.1016/j.cell.2015.04.017

Figure 1.

Figure 1

MCX1 interacts chemically and genetically with the heme biosynthetic pathway. (A) The metabolic pathway for the first step of heme biosynthesis in non-plant eukaryotes. The genetic and chemical interaction profile of MCX1 is highly correlated with the profiles of yeast genes (HEM25, HEM1, and HEM2, shown in red) involved in the first steps of heme biosynthesis. Dashed lines indicate uncertainty in assigning Hem25 to glycine uptake or ALA export. Gray bars indicate mitochondrial membranes. See Fig. S1 for alignment showing conservation among ClpX homologs. (B) MCX1, HEM1, and HEM25 alleles exhibit synthetic phenotypes. Five-fold serial dilutions from cell suspensions with OD600 = 1 were pinned on YP + 2% agar, + 2% glucose or 3% glycerol. +ALA indicates 50 μg/mL ALA. Growth on glucose after 2 d and on glycerol after 3 d is shown.